As the first frequency domain (FD) FLIM camera using a two readout-tap per pixel CMOS sensor, our pco.flim offers synchronized modulation of pixels and stimulated light which enables the analysis of luminescence decay times in the range of sub nanoseconds to microseconds. With its 1008 x 1008 pixels resolution, the pco.flim reads out 45 double frames/s at a maximum and can be used for a modulation frequency range of 5 kHz – 40 MHz. Flexible coupling and easy setup makes this camera an ideal choice for the widefield FD-FLIM instruments.
The pco.flim camera system offers flexible coupling to any microscope or lens via a C-mount and includes a USB 3.0 interface to easily connect the camera to a variety of computers. The pco.flim laser is a homogeneously illuminating light source designed for use with the pco.flim. It features a digital modulation frequency range of 0 – 250 MHz. The pco.flim laser is suitable for wide-field epifluorescence microscope illumination, Total-Internal-Reflection-Fluorescence (TIRF) microscopy, light sheet fluorescence microscopy (LSFM) and confocal spinning disk microscopy. Choose between a wide range of different wavelengths and light output powers. The pco.flim laser is ideally suited for use with relevant luminescence lifetimes in the range of sub nanoseconds to microseconds. For those using a Nikon microscope, a Nikon double-safety-shutter option is also available.
Also, try our MachVis Lens Selector tool to help identify the perfect lens solution and PCO camera for your imaging and machine vision requirements.
Video: Frequency Domain Fluorescence Lifetime Imaging Microscopy - A Hands-On Session
This hands-on session about FD-FLIM by Gerhard Holst from Excelitas' PCO brand was given as part of the FLIM Symposium "Beginners Guide to Fluorescence Lifetime Imaging in Life Sciences."
The online FLIM symposium was co-organized by CMCB Light Microscopy Facility of Technische Universität Dresden, Imaging Network of WWU Münster, Center of Advanced Imaging of HHU Düsseldorf University and the Core Facility Bioimaging at the Biomedical Center of the Ludwig-Maximilians-Universität München.
Video: Frequency Domain Fluorescence Lifetime Imaging Microscopy - A Hands-On Session
Video: Fluorescence Lifetime Imaging FLIM with the frequency domain pco flim system
This video tutorial explains how fluorescence lifetime imaging (FLIM) can be done and the benefit of frequency domain FLIM done with a pco.flim camera system.
