pco.edge 9.4 bi CLHS sCMOS Camera
The pco.edge 9.4 bi CLHS is our first sCMOS camera with photon counting capabilities for challenging and demanding microscope methods like Lightsheet Fluorescence Microscopy (LSFM) and Structured Illumination Microscopy (SIM). This sCMOS camera combines an extremely low readout noise of 0.3 e- with a quantum efficiency of up to 85% with a broad spectrum out to NIR. The sensor incorporates microlenses and a full pixel height deep trench isolation for crosstalk suppression, resulting in an excellent modulation transfer function. The camera provides a large image circle using a high-resolution 9.4MPixel image sensor with a square pixel size of 4.6µm. The camera is equipped with four CLHS FOL channels – all aligned in one compact plug – capable of transmitting up to 4.9 GByte/s.
With low readout noise values, the pco.edge 9.4 bi CLHS can capture light with photon counting sensitivity and use even the smallest signals to gain information about the imaged samples. With its image sensor diagonal of 21.6 mm the camera offers a large image circle suitable for a wide range of applications in microscopy. Due to its rectangular layout, the pco.edge 9.4 bi CLHS can also be used for two-color applications that use image splitters.
| Length | 109.0 mm |
| Width | 90.0 mm |
| Height | 95.0 mm |
| Weight (kg) | 1.35 |
| Dynamic Range (log) |
87.0 dB 84.0 dB 82.0 dB |
| Forced Air | true |
| Readout Noise RMS |
0.4 e- 0.5 e- 0.6 e- |
| Pixel Size (H x V) | 4.6 x 4.6 |
| Thermo Electric Cooling | true |
| Frame rate (Full Sensor) |
30.0 fps 60.0 fps 122.0 fps |
| Peak Quantum Efficiency | 85.0 % |
| Dark Current | 0.4 e-/s |
| Working Temperature Image Sensor |
adjustable: 0 °C to +25 °C calibration setpoint: +10 °C |
| Peak Quantum Efficiency Wavelength | 500.0 nm |
| Readout Noise Median |
0.3 e- 0.4 e- 0.5 e- |
| Sensor Technology | sCMOS BSI |
| Sensor Version | HWK4123 |
| Fullwell Capacity |
7000 e- 6500 e- 6500 e- |
| Sensor Temperature (Dark Current Reference) | 10.0 °C |
| Operational Modes |
Slow Scan Medium Scan Fast Scan |
| Dynamic Range A/D Converter | 16 bit |
| Dynamic Range |
23300:1 16200:1 13000:1 |
| Resolution (H x V) | 4096 x 2300 |
| Sensor Diagonal | 21.6 mm |
| Liquid Cooling | true |
| Included Lens Mount |
C-Mount F-Mount |
| Data Interface | Camera Link HS FOL |
| Line Scanning | true |
| Global Reset | false |
| Trigger Input Signals |
external exposure start external exposure control acquire enable |
| Global Shutter | false |
| Region of Interest Vertical (ROI) | vertical: steps of 4 rows |
| Non Linearity | 0.3 % |
| MTF (Theoretical) | 108.6 lp/mm |
| Optional Lens Mount |
TFL-Mount EF-Mount |
| Spectral Range Minimum | 400 nm |
| Dark Signal Non-Uniformity DSNU |
≤ 0.05 e- (rms) ≤ 0.1 e- (rms) ≤ 0.1 e- (rms) |
| Binning Vertical |
x2 x4 |
| Bining Method |
average sum |
| Binning Horizontal |
x2 x4 |
| Default Read Out Mode | Slow Scan |
| Operating Temperature Range | +10 °C to + 40 °C |
| Photo Response Non-Uniformity PRNU |
≤ 0.1 % ≤ 0.2 % ≤ 0.2 % |
| Sensor Manufacturer | Fairchild Imaging |
| Power Supply | 24 VDC (±10 %) |
| Exposure Minimum |
29 μs 15 μs 7 μs |
| Sensor Dimensions (H x V) | 18.8 x 10.6 |
| Spectral Range Maximum | 1050 nm |
| A/D Conversion Factor |
0.11 e-/DN 0.1 e-/DN 0.1 e-/DN |
| Direct Mounting Distance (No Camera Flange) | 6.2 mm (±10 %) |
| Chroma | mono |
| Rolling Shutter | true |
| Humidity Range (Non-Condensing) | 10 % to 80 % (recommended < 65 %) |
| Trigger Cable Connector | SMA |
| Double Shutter | false |
| Read Out Mode: Set Parameter | Pixel Rate |
| Power Consumption | max. 40 W |
| Storage Temperature Range | -10 °C to +60 °C |
| Pixel Rate |
367 MPixel/s 734 MPixel/s 1467 MPixel/s |
| Exposure Maximum |
10 s 10 s 10 s |
| Compliance Label |
CE FCC UKCA |
| Region of Interest Horizontal (ROI) | horizontal: steps of 32 columns |
| Trigger Output Signals |
exposure busy line |
| Part Number | 85108076030 |
- Brightfield microscopy
- Fluorescence microscopy
- Digital pathology
- Single molecule localization microscopy (SMLM)
- Light sheet fluorescence microscopy (LSFM)
- Selective plane illumination microscopy (SPIM)
- Structured illumination microscopy (SIM)
- Raman spectroscopy
- Calcium imaging
- Förster resonance energy transfer (FRET)
- Fluorescence recovery after photobleaching (FRAP)
- High-speed bright field ratio imaging
- High throughput screening
- Opthalmology
- Biochip reading
- Total internal reflection fluorescence microscopy (TIRF)
- 3D metrology
- Industrial quality inspection
- Wafer inspection
- Image intensifier imaging
- Intravital microscopy
- Inspection
- Material testing
- Biometrics
- In-vivo microscopy
| Length | 109.0 mm |
| Width | 90.0 mm |
| Height | 95.0 mm |
| Weight (kg) | 1.35 |
| Dynamic Range (log) |
87.0 dB 84.0 dB 82.0 dB |
| Forced Air | true |
| Readout Noise RMS |
0.4 e- 0.5 e- 0.6 e- |
| Pixel Size (H x V) | 4.6 x 4.6 |
| Thermo Electric Cooling | true |
| Frame rate (Full Sensor) |
30.0 fps 60.0 fps 122.0 fps |
| Peak Quantum Efficiency | 85.0 % |
| Dark Current | 0.4 e-/s |
| Working Temperature Image Sensor |
adjustable: 0 °C to +25 °C calibration setpoint: +10 °C |
| Peak Quantum Efficiency Wavelength | 500.0 nm |
| Readout Noise Median |
0.3 e- 0.4 e- 0.5 e- |
| Sensor Technology | sCMOS BSI |
| Sensor Version | HWK4123 |
| Fullwell Capacity |
7000 e- 6500 e- 6500 e- |
| Sensor Temperature (Dark Current Reference) | 10.0 °C |
| Operational Modes |
Slow Scan Medium Scan Fast Scan |
| Dynamic Range A/D Converter | 16 bit |
| Dynamic Range |
23300:1 16200:1 13000:1 |
| Resolution (H x V) | 4096 x 2300 |
| Sensor Diagonal | 21.6 mm |
| Liquid Cooling | true |
| Included Lens Mount |
C-Mount F-Mount |
| Data Interface | Camera Link HS FOL |
| Line Scanning | true |
| Global Reset | false |
| Trigger Input Signals |
external exposure start external exposure control acquire enable |
| Global Shutter | false |
| Region of Interest Vertical (ROI) | vertical: steps of 4 rows |
| Non Linearity | 0.3 % |
| MTF (Theoretical) | 108.6 lp/mm |
| Optional Lens Mount |
TFL-Mount EF-Mount |
| Spectral Range Minimum | 400 nm |
| Dark Signal Non-Uniformity DSNU |
≤ 0.05 e- (rms) ≤ 0.1 e- (rms) ≤ 0.1 e- (rms) |
| Binning Vertical |
x2 x4 |
| Bining Method |
average sum |
| Binning Horizontal |
x2 x4 |
| Default Read Out Mode | Slow Scan |
| Operating Temperature Range | +10 °C to + 40 °C |
| Photo Response Non-Uniformity PRNU |
≤ 0.1 % ≤ 0.2 % ≤ 0.2 % |
| Sensor Manufacturer | Fairchild Imaging |
| Power Supply | 24 VDC (±10 %) |
| Exposure Minimum |
29 μs 15 μs 7 μs |
| Sensor Dimensions (H x V) | 18.8 x 10.6 |
| Spectral Range Maximum | 1050 nm |
| A/D Conversion Factor |
0.11 e-/DN 0.1 e-/DN 0.1 e-/DN |
| Direct Mounting Distance (No Camera Flange) | 6.2 mm (±10 %) |
| Chroma | mono |
| Rolling Shutter | true |
| Humidity Range (Non-Condensing) | 10 % to 80 % (recommended < 65 %) |
| Trigger Cable Connector | SMA |
| Double Shutter | false |
| Read Out Mode: Set Parameter | Pixel Rate |
| Power Consumption | max. 40 W |
| Storage Temperature Range | -10 °C to +60 °C |
| Pixel Rate |
367 MPixel/s 734 MPixel/s 1467 MPixel/s |
| Exposure Maximum |
10 s 10 s 10 s |
| Compliance Label |
CE FCC UKCA |
| Region of Interest Horizontal (ROI) | horizontal: steps of 32 columns |
| Trigger Output Signals |
exposure busy line |
| Part Number | 85108076030 |
- Brightfield microscopy
- Fluorescence microscopy
- Digital pathology
- Single molecule localization microscopy (SMLM)
- Light sheet fluorescence microscopy (LSFM)
- Selective plane illumination microscopy (SPIM)
- Structured illumination microscopy (SIM)
- Raman spectroscopy
- Calcium imaging
- Förster resonance energy transfer (FRET)
- Fluorescence recovery after photobleaching (FRAP)
- High-speed bright field ratio imaging
- High throughput screening
- Opthalmology
- Biochip reading
- Total internal reflection fluorescence microscopy (TIRF)
- 3D metrology
- Industrial quality inspection
- Wafer inspection
- Image intensifier imaging
- Intravital microscopy
- Inspection
- Material testing
- Biometrics
- In-vivo microscopy
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